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KMID : 1145120180160010131
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Asian Journal of Beauty and Cosmetology
2018 Volume.16 No. 1 p.131 ~ p.138
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Inhibition Effect of Cnidium officinale Makino Extracts on MMP1 Expression in Human Dermal Fibroblasts
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Kim Young-Joo
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Abstract
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Purpose: Cnidium officinale Makino (C. officinale) belonged to Umbelliferae family is medical herb native to China and is extensively cultivated in Korea, China and Japan. C. officinale has been used to oriental medicine against pain, inflammation, menstrual disturbance, and anti-vitamin deficiency disease, and also acts as a blood pressure depressant. However, effects of C. officinale are not fully understood in dermis. Therefore, in this study, we identified effects of C. officinale in dermal fibroblast.
Methods: Matrix metalloproteinase-1 (MMP1) expression of C. officinale extract was valuated using quantitative real time polymerase chain reaction (qRT-PCR). In addition, transcriptional activity of activator protein 1 (AP-1) and c-Jun N-terminal protein kinase (JNK) activity were assayed using luciferase assay and western blot.
Results: Extracts of C. officinale decreased MMP1 expression in ultraviolet (UV)-exposed human dermal fibroblasts. Moreover, C. officinale extracts showed decrease of AP-1 transcriptional activity and phospho-JNK in UV-exposed human dermal fibroblasts.
Conclusion: Our results suggest that C. officinale extracts decreased MMP1 expression using decreasing AP-1 transcriptional activity and phospho-JNK. Therefore, C. officinale extracts has potential to reduce formation of wrinkle and to use as a cosmetic ingredient.
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KEYWORD
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MMPs, AP-1, JNK, Cnidium officinale Makino, UV
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